PHENOTYPIC AND FUNCTIONAL DIFFERENCES BETWEEN HUMAN DENDRITIC CELLS DERIVED IN-VITRO FROM HEMATOPOIETIC PROGENITORS AND FROM MONOCYTES MACROPHAGES

We compared dendritic cells (DC) derived from CD34(+) hematopoietic pr ogenitor cells with tumor necrosis factor alpha and granulocyte-macrop hage colony-stimulating factor (GM-CSF) to DC derived from monocytes/m acrophages with interleukin-4 (IL-4) and GM-CSF, Monocyte/macrophage - derived DC demonstrated higher levels of CD1a, lower levels of CD14, greater stimulatory activity in mixed lymphocyte reactions, and greate r capacity to present soluble protein antigen than CD34(+) cell-derive d DC, Lymphocytes stimulated with antigen-pulsed, monocyte/macrophage derived DC produced more IL-10 than those stimulated with antigen-puls ed, CD34(+)-derived DC, Whereas CD1a(+) DC could be derived from CD34( +) cells in serum-free- and human-sera-containing cultures, the deriva tion of CD1a(+) DC from monocytes/macrophages required the presence of fetal calf serum, The spectrum of cytokine mRNA expression, the prese ntation of peptide antigen, and the sensitivity to human immune defici ency virus-1 infection of CD34(+)- and monocyte/ macrophage-derived DC were comparable, Although cells derived by both methods are potent an tigen presenting cells, there are differences between DC derived in vi tro from hematopoietic progenitors and from monocytes/macrophages that may influence their in vivo activity.