ASSESSMENT OF ENZYME-LINKED-IMMUNOSORBENT-ASSAY BASED DIAGNOSTIC KITS(TOXOKIT-G AND TOXOKIT-M) FOR THE DETECTION OF IGG AND IGM ANTIBODIESTO TOXOPLASMA-GONDII IN HUMAN SERUM
Gm. Bhopale et al., ASSESSMENT OF ENZYME-LINKED-IMMUNOSORBENT-ASSAY BASED DIAGNOSTIC KITS(TOXOKIT-G AND TOXOKIT-M) FOR THE DETECTION OF IGG AND IGM ANTIBODIESTO TOXOPLASMA-GONDII IN HUMAN SERUM, Comparative immunology, microbiology and infectious diseases, 20(4), 1997, pp. 309-314
An enzyme linked immunosorbent assay (ELISA) using penicillinase was d
eveloped in the form of diagnostic kits (Toxokit-G and Toxokit-M) for
the detection of IgG and IgM antibodies to Toxoplasma gondii. The perf
ormance of both the kits was compared with commercially available diag
nostic kits, i.e. Enzygnost-Toxoplasmosis/IgG (Behring Co., Germany),
TOXOTEK-G (Flow Lab., U.K.) and Toxoplasma IgM Microassay (Diamedix Co
rp., U.S.A.) by testing toxoplasma-suspected human serum samples. The
results indicate a good reliability between these diagnostic kits. Tox
okit-G has 86.66 and 96.05% sensitivity and specificity respectively.
The main advantage of Toxokit-G is that the end result can be assessed
visually without using sophisticated instruments. Toxokit-M has 100%
sensitivity and specificity and test results were not affected by the
presence of antitoxoplasma IgG antibodies, rheumatoid factor or antinu
clear antibodies. Published by Elsevier Science Ltd.