H. Ellakany et al., IMMUNOBLOT EXAMINATION OF HUMORAL RESPONSE OF CHICKENS INFECTED WITH MYCOPLASMA-GALLISEPTICUM AT VARIOUS AGES, Comparative immunology, microbiology and infectious diseases, 20(4), 1997, pp. 319-333
Mycoplasma gallisepticum and Mycoplasma synoviae-free chickens were in
fected with 0.2 mi broth culture of M. gallisepticum strain 1226 intra
air sac at 3, 14, 18, 28, 42, 49 and 65 days of age. Blood samples we
re taken 0-5 weeks before infection and 1-6 weeks after infection (dep
ending on age of infection). The antibody response was examined by Wes
tern blot. As a control of infection, serum plate agglutination test (
SPA), pathological lesions, and presence of Mycoplasma in air sacs wer
e used. Antibodies to p64-67 kDa appeared in all groups of birds on th
e first week post-infection. Antibodies to p56 were detected from the
second week post-challenge if infection was performed at 3 or 14 days
of age, while on first week if challenge was done at 18, 28, 42, 49 or
65 days of age. Antibodies to p200, p120, p98, p80, p75, p72. p60, p5
0, p45, p40, p35, p33, p31, p28, p26, p24 and p22 were also detected.
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