A BETA-LACTAMASE BELONGING TO GROUP 2E FROM ORAL CLINICAL ISOLATES OFPREVOTELLA-INTERMEDIA

A beta-lactamase in oral clinical isolates of Prevotella intermedia th at hydrolyzed cefuroxime and cephalothin with rates of 600 and 53.3 re spectively, relative to that for cephaloridine (100), was characterize d as a 2e-cephalosporinase. Inhibition was observed by clavulanic acid (IC50 0.72 mu M), tazobactam (IC50 0.21 mu M) and sulbactam (IC50 0.0 7 mu M) and was not inhibited by cloxacillin, EDTA, NaCl or p-CMB. The pI and pH optima were 4.7 and 5.4, respectively. (C) 1998 Federation of European Microbiological Societies. Published by Elsevier Science B .V.