THE PERIPHERAL-MYELIN-PROTEIN-22 KDA (PMP22) GENE IS AMPLIFIED IN CELL-LINES DERIVED FROM GLIOMA AND OSTEOGENIC-SARCOMA - A NEW CANDIDATE FOR AN ONCOGENE
T. Liehr et al., THE PERIPHERAL-MYELIN-PROTEIN-22 KDA (PMP22) GENE IS AMPLIFIED IN CELL-LINES DERIVED FROM GLIOMA AND OSTEOGENIC-SARCOMA - A NEW CANDIDATE FOR AN ONCOGENE, International journal of oncology, 10(5), 1997, pp. 915-919
Two glioma (SF188, SF763) and two osteogenic sarcoma cell lines (RH30,
SA1) were examined for the presence of an amplification of the PMP22
gene by means of fluorescence in situ hybridization (FISH). In one cel
l line of both cell types, we found about 10 copies of the PMP22 (peri
pheral myelin protein 22 kDa) gene, located on different marker chromo
somes within homogeneously staining regions. Surrounding chromosome 17
p material was found to be coamplified, but coamplification of TP53 (1
7p13) and erbB2/Her2 (17q11.12) were excluded by FISH for both cell li
nes, SF763 and RH30. This is the first report of a PMP22 amplification
in cell lines derived from human tumors.