ENANTIOMERIC SEPARATION OF PROPRANOLOL AND SELECTED METABOLITES BY USING CAPILLARY ELECTROPHORESIS WITH HYDROXYPROPYL-BETA-CYCLODEXTRIN AS CHIRAL SELECTOR

A capillary electrophoresis (CE) investigation of the enantiomeric sep aration of propranolol and some of its metabolites using CE was undert aken. Resolution of the enantiomers was achieved using hydroxypropyl-b eta-cyclodextrin (HP-beta-CD) as the chiral selector. Parameters found to influence separation include cyclodextrin concentration, potential , pH and organic solvent/additive. It was observed that 17 mM HP-beta- CD gave optimum separation over the concentration range used in this s tudy, however different racemates appear to have best resolution at di fferent CD concentration. The potential does not have a great effect o n enantiomer resolution, but appears to cause relative metabolite migr ation times to alter such that separation is affected. Carrier pH affe cts both migration time, and enantiomer resolution and metabolite sepa ration. Above pH 5 inferior results are obtained. This is the first re port of enantiomeric resolution of propranolol metabolites using CE. ( C) 1998 Elsevier Science B.V.