M. Keinanen et R. Julkunentiitto, HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF FLAVONOIDS IN BETULA-PENDULA AND BETULA-PUBESCENS LEAVES, Journal of chromatography, 793(2), 1998, pp. 370-377
Citations number
36
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
A high-performance liquid chromatography (HPLC) procedure based on a w
ater-methanol gradient with tetrahydrofuran for simultaneous, analysis
of flavonoids and other phenolics of differing polarities in birch le
aves was developed. Mobile phases with and without tetrahydrofuran or
orthophosphoric acid provided additional information for tentative ide
ntification of the compounds. Forty-five compounds were determined fro
m Betula pendula and B. pubescens leaves. Five of the isolated flavono
l glycosides have nor been reported earlier from B. pendula. B. pendul
a leaves have a range of myricetin and quercetin glycosides with the s
ame sugar moieties in roughly the same proportions. Flavonoid aglycone
s were deposited on leaf surfaces. (C) 1998 Elsevier Science B.V.