STUDIES OF THE RATE-CONSTANT OF SUCROSE H YDROLYSIS BY CAPILLARY ELECTROPHORESIS WITH ELECTROCHEMICAL DETECTION

The concentrations of sucrose and its hydrolysis products were directl y monitored by capillary electrophoresis-electrochemical detection due to its high resolution and high sensitivity of detection, The rate co nstants of sucrose hydrolysis at different temperatures in 0.80 mol/L HCl and 0.90 mol/L HCl were obtained from the concentration change of sucrose during the process of hydrolysis, At 25 degrees C, 30 degrees C and 35 degrees C, the measured rate constants of sucrose hydrolysis were 7.81 X 10(-3) min(-1), 17.44 X 10(-3) min(-1) and 33.09 X 10(-3) min(-1) in 0.80 mol/L HCl, and 11.18 X 10(-3) min(-1), 27.32 X 10(-3) min(-1) and 44.86 X 10(-3) min(-1) in 0.90 mol/L HCl, respectively, fr om which the E-a value of 109.85 kJ/mol was derived. Compared with the traditional polarimetric method, this method is more visualizable and convenient with satisfactory results.