HIGH-RESOLUTION YAC-COSMID-STS MAP OF HUMAN-CHROMOSOME-13

We have assembled a high-resolution physical map of human chromosome 1 3 DNA (similar to 114 Mb) from hybridization, PCR, and FTSH mapping da ta using a specifically designed set of computer programs. Although th e mapping of 13p is limited, 13q (similar to 98 Mb) is covered by an a lmost continuous contig of 736 YACs aligned to 597 contigs of cosmids. Of a total of 10,789 cosmids initially selected from a chromosome 13- specific cosmid library (16,896 colonies) using inter-Alu PCR probes f rom the YACs and probes for markers mapped to chromosome 13, 5111 were assembled in contigs that were established from cross-hybridization r elationships between the cosmids. The 13q YAC-cosmid map was annotated with 655 sequence tagged sites (STSs) with an average spacing of 1 ST S per 150 kb. This set of STSs, each identified by a D number and cyto genetic location, includes database markers (198), expressed sequence tags (93), and STSs generated by sequencing of the ends of cosmid inse rts (364). Additional annotation has been provided by positioning 197 cosmids mapped by FISH on 13q. The final (comprehensive) map, a list o f STS primers, and ran; data used in map assembly are available at our Web site (genome1.ccc.columbia.edu/similar to genome/) and can serve as a resource to facilitate accurate localization of additional marker s, provide substrates for sequencing, and assist in the discovery of c hromosome 13 genes associated with hereditary diseases. (C) 1999 Acade mic Press.