MAPPING OF THE HUMAN GENES ENCODING CYCLIN-H (CCNH) AND THE CDK-ACTIVATING KINASE (CAK) ASSEMBLY FACTOR MAT1 (MNAT1) TO CHROMOSOME BANDS 5Q13.3-Q14 AND 14Q23, RESPECTIVELY

Cyclin-dependent kinases (CDKs), which play a key role in cell cycle c ontrol, are activated by the CDK-activating kinase (CAK), which activa tes cyclin-bound CDKs by phosphorylation at the specific threonine res idue. Mammalian CAK contains three components: CDK7, cyclin H, and an assembly factor called MAT1. The CDK7-cyclin H-MAT1 complex is tightly associated with a multiprotein complex TFIIH, which plays a dual role in transcription and DNA repair. Here, we have determined chromosomal localizations of the human genes encoding cyclin H (CCNH) and MAT1 (H GMW-approved symbol MNAT1) to chromosome bands 5q13.3-q14 and 14q23, r espectively, by using fluorescence in situ hybridization, somatic cell hybrid analyses, and mapping to the human YAC contigs. (C) 1998 Acade mic Press.