Va. Tkachuk et al., IDENTIFICATION OF AN ATYPICAL LIPOPROTEIN-BINDING PROTEIN FROM HUMAN AORTIC SMOOTH-MUSCLE AS T-CADHERIN, FEBS letters, 421(3), 1998, pp. 208-212
We have previously described an atypical lipoprotein-binding protein o
f about 105 kDa (p105) in membranes of vascular smooth muscle cells (V
SMCs) that is distinct from currently known lipoprotein receptors. In
the present work we have developed a procedure for purification of p10
5 from human aortic media. Partial sequencing of purified protein has
revealed identity of p105 with human T-cadherin. Anti-peptide antisera
raised against human T-cadherin recognized a protein spot correspondi
ng to the purified p105 on two-dimensional Western blots. The antisera
also inhibited LDL binding to p105 on ligand blots. We conclude that
the 105 kDa lipoprotein-binding protein present in human VSMCs is T-ca
dherin, an unusual glycosylphosphatidylinositol-anchored member of the
cadherin family of cell-cell adhesion proteins. (C) 1998 Federation o
f European Biochemical Societies.