NOVEL SUBTYPING OF INTESTINAL METAPLASIA IN THE HUMAN STOMACH - BRAIN-TYPE GLYCOGEN-PHOSPHORYLASE EXPRESSION IN THE PROLIFERATIVE ZONE AND ITS RELATIONSHIP WITH CARCINOGENESIS
H. Matsuzaki et al., NOVEL SUBTYPING OF INTESTINAL METAPLASIA IN THE HUMAN STOMACH - BRAIN-TYPE GLYCOGEN-PHOSPHORYLASE EXPRESSION IN THE PROLIFERATIVE ZONE AND ITS RELATIONSHIP WITH CARCINOGENESIS, AJCP. American journal of clinical pathology, 109(2), 1998, pp. 181-189
Although reports have suggested the incomplete type of intestinal meta
plasia (IM) had a close correlation with carcinoma; considerable data
showed no apparent relationship between the particular type of IM and
the intestinal type carcinoma. The purpose of this study was to establ
ish a novel classification of IM using brain-type glycogen phosphoryla
se (BGP) from a carcinogenetic viewpoint. The only isoform expressed i
n gastric cancer was BGP using polymerase chain reaction analysis. We
studied 136 specimens with gastric carcinoma and the adjacent IM using
specific anti-BGP antibody with its correlation to subtypes of IM, pr
oliferating cell nuclear antigen-labeling index, and various oncogene
products. Brain-type glycogen phosphorylase was expressed in 80.5% of
the intestinal type and 18.8% of the diffuse type of carcinoma and in
87.5% and 41.6% in the generative zone of IM adjacent to cancer foci,
respectively, whereas no reactivity was observed in the normal gastric
mucosa. The proportion of the positivity in the cancer and IM was sig
nificantly greater in the intestinal-type carcinoma than in the diffus
e type. The expression of BGP in the generative cells of IM had no sig
nificant correlation with the conventional type of IM. Intestinal meta
plasias with BGP expression were significantly higher in a proliferati
ng state than in those without BGP, and some of them that were coexpre
ssed accumulated p53 in the generative cells. The relationship between
IM with BGP in the generative cells and intestinal-type carcinoma was
apparently closer than the conventional subtype of IM and gastric can
cer. Intestinal-type carcinoma might arise from some of these prolifer
ating cells with BGP.