A REPETITIVE DNA-SEQUENCE COMMON TO THE DIFFERENT B-CHROMOSOME OF THEGENUS BRACHYCOME

Citation
A. Houben et al., A REPETITIVE DNA-SEQUENCE COMMON TO THE DIFFERENT B-CHROMOSOME OF THEGENUS BRACHYCOME, Chromosoma, 106(8), 1997, pp. 513-519
Citations number
37
Categorie Soggetti
Genetics & Heredity",Biology
Journal title
ISSN journal
00095915
Volume
106
Issue
8
Year of publication
1997
Pages
513 - 519
Database
ISI
SICI code
0009-5915(1997)106:8<513:ARDCTT>2.0.ZU;2-F
Abstract
Dot-like micro B chromosomes of Brachycome dichromosomatica were analy sed for their sequence composition. Southern hybridization patterns of a total micro B probe to genomic DNA from plants with and without mic ro Bs demonstrated that the micro Bs shared sequences with the A chrom osomes. In addition to telomere, rDNA and common A and B chromosome se quences, a new B-specific, highly methylated tandem repeat (Bdm29) was detected. After in situ hybridization with Bdm29 the entire micro B c hromosome was labelled and clustering of the condensed micro Bs could be observed at interphase. A high number of Bdm29-like sequences were also found in the larger B chromosomes of B. dichromosomatica and in o ther Bs within the genus Brachycome.