DIETARY-FAT AND BREAST-CANCER METASTASIS BY HUMAN TUMOR XENOGRAFTS

Citation
Dp. Rose et Jm. Connolly, DIETARY-FAT AND BREAST-CANCER METASTASIS BY HUMAN TUMOR XENOGRAFTS, Breast cancer research and treatment, 46(2-3), 1997, pp. 225-237
Citations number
72
Categorie Soggetti
Oncology
ISSN journal
01676806
Volume
46
Issue
2-3
Year of publication
1997
Pages
225 - 237
Database
ISI
SICI code
0167-6806(1997)46:2-3<225:DABMBH>2.0.ZU;2-Q
Abstract
Human breast cancer cell Lines growing as xenografts in athymic nude m ice have been used to examine the effects of dietary fat and fatty aci ds on tumor progression. The estrogen independent MDA-MB-435 cell line has the advantage that it metastasizes consistently to the lungs and forms quantifiable secondary nodules when injected into the mammary fa t pads. With these breast cancer cells, the stimulating effects of pol yunsaturated omega-6 fatty acids on both primary tumor growth and meta stasis were demonstrated; in contrast, the long-chain omega-3 fatty ac ids were inhibitory. The model can also be adapted to examine dietary fatty acids, and inhibitors of their metabolism, as experimental adjuv ant therapy after surgical excision of the primary tumors. Unfortunate ly, estrogen dependent human breast cancer cells do not metastasize, o r do so rarely, in nude mice; in consequence, it is not possible to us e the model to study estrogen-fatty acid interactions on the metastati c process. In addition to metastasis from a primary location, intraven ous injection of MDA-MB-435 cells into the nude mouse host, particular ly when combined with studies using Matrigel-based in vitro invasion a ssays, permits further dissection of the steps in the metastatic casca de which are influenced by dietary fatty acids. The results obtained b y these several approaches have demonstrated distinct roles for the cy clooxygenase and lipoxygenase-mediated products of omega-6 fatty acid metabolism, and suggest new approaches to experimental breast cancer t herapy.