HUMAN RPA (HSSB) INTERACTS WITH EBNA1, THE LATENT ORIGIN-BINDING PROTEIN OF EPSTEIN-BARR-VIRUS

Authors
ZHANG D FRAPPIER L GIBBS E HURWITZ J ODONNELL M
Citation
D. Zhang et al., HUMAN RPA (HSSB) INTERACTS WITH EBNA1, THE LATENT ORIGIN-BINDING PROTEIN OF EPSTEIN-BARR-VIRUS, Nucleic acids research, 26(2), 1998, pp. 631-637
Citations number
41
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
26
Issue
2
Year of publication
1998
Pages
631 - 637
Database
ISI
SICI code
0305-1048(1998)26:2<631:HR(IWE>2.0.ZU;2-Z
Abstract
RPA is the replicative single-strand DNA (ssDNA) binding protein of eu karyotic chromosomes. This report shows that human RPA interacts with EBNA1, the latent origin binding protein of Epstein-Barr virus (EBV), RPA binds to EBNA1 both in solution, and when EBNA1 is bound to the EB V origin. RPA is a heterotrimer, and the main contact with EBNA1 is fo rmed through the 70 kDa subunit of RPA, the subunit which binds to ssD NA, We propose that this interaction between RPA and EBNA1 is an early step in activation of the latent origin of EBV.