T. Dewit et al., MICROINJECTION OF CRE RECOMBINASE RNA INDUCES SITE-SPECIFIC RECOMBINATION OF A TRANSGENE IN MOUSE OOCYTES, Nucleic acids research, 26(2), 1998, pp. 676-678
We have developed a strategy for producing single copy transgenic mous
e lines using Cre-loxP site specific recombination. The method is base
d on transient expression of the recombinase after injection of in vit
ro transcribed mRNA into the cytoplasm of fertilised eggs containing m
ultiple copies of the transgene, The success rate of the recombination
event is 100% (15 out of 15).