MICROINJECTION OF CRE RECOMBINASE RNA INDUCES SITE-SPECIFIC RECOMBINATION OF A TRANSGENE IN MOUSE OOCYTES

Authors
DEWIT T DRABEK D GROSVELD F
Citation
T. Dewit et al., MICROINJECTION OF CRE RECOMBINASE RNA INDUCES SITE-SPECIFIC RECOMBINATION OF A TRANSGENE IN MOUSE OOCYTES, Nucleic acids research, 26(2), 1998, pp. 676-678
Citations number
18
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
26
Issue
2
Year of publication
1998
Pages
676 - 678
Database
ISI
SICI code
0305-1048(1998)26:2<676:MOCRRI>2.0.ZU;2-C
Abstract
We have developed a strategy for producing single copy transgenic mous e lines using Cre-loxP site specific recombination. The method is base d on transient expression of the recombinase after injection of in vit ro transcribed mRNA into the cytoplasm of fertilised eggs containing m ultiple copies of the transgene, The success rate of the recombination event is 100% (15 out of 15).