DESIGNER DELETION STRAINS DERIVED FROM SACCHAROMYCES-CEREVISIAE S288C- A USEFUL SET OF STRAINS AND PLASMIDS FOR PCR-MEDIATED GENE DISRUPTION AND OTHER APPLICATIONS

A set of yeast strains based on Saccharomyces cerevisiae S288C in whic h commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. These st rains minimize or eliminate the homology to the corresponding marker g enes in commonly used vectors without significantly affecting adjacent gene expression. Because the homology between commonly used auxotroph ic marker gene segments and genomic sequences has been largely or comp letely abolished, these strains will also reduce plasmid integration e vents which can interfere with a wide variety of molecular genetic app lications. We also report the construction of new members of the pRS40 0 series of vectors, containing the kanMX, ADE2 and MET15 genes. (C) 1 998 John Wiley & Sons, Ltd.