Development of red-brown pigments typical for Tilsit cheese by smear b
acteria was studied in milk-based model systems. The yellow pigmented
Arthrobacter strain CA12 was found to be essential. Pure milk cultures
of strain CA12 developed an extracellular yellow color. Addition of c
asein hydrolysate led to a production of red brown color by the same s
train. The production of red-brown pigments could not be reproduced by
the addition of single amino acids. In mixed culture of Arthrobacter
strain CA12 with proteolytic Brevibacterium linens strains, the same r
ed-brown color was produced. This was independent of the pigmentation
of the Brevibacterium strain used for the experiments. Absorbance spec
tra showed that the red-brown color was related to the original Arthro
bacter yellow pigments.