NUCLEOTIDE AND AMINO-ACID-SEQUENCES OF THE METALLO-BETA-LACTAMASE, IMIS, FROM AEROMONAS-VERONII BV SOBRIA

The Aeromonas veronii by. sobria metallo-a-lactamase gene, imiS, was c loned. The imiS open reading frame extends for 762 bp and encodes a pr otein of 254 amino acids with a secreted modified protein of 227 amino acids and a predicted pi of 8.1. To confirm the predicted sequence, p urified ImiS was digested and the resulting peptides were identified, yielding an identical sequence for ImiS, with 98% identity to CphA. Bo th possessed the putative active-site sequence Asn-Tyr-His-Thr-Asp at positions 88 to 92, which is unique to the Aeromonas metallo-beta-lact amases.