THE CYS607-]TYR CHANGE IN THE UL97 PHOSPHOTRANSFERASE CONFERS GANCICLOVIR RESISTANCE TO 2 HUMAN CYTOMEGALOVIRUS STRAINS RECOVERED FROM 2 IMMUNOCOMPROMISED PATIENTS

Authors
BALDANTI F UNDERWOOD MR TALARICO CL SIMONCINI L SARASINI A BIRON KK GERNA G
Citation
F. Baldanti et al., THE CYS607-]TYR CHANGE IN THE UL97 PHOSPHOTRANSFERASE CONFERS GANCICLOVIR RESISTANCE TO 2 HUMAN CYTOMEGALOVIRUS STRAINS RECOVERED FROM 2 IMMUNOCOMPROMISED PATIENTS, Antimicrobial agents and chemotherapy, 42(2), 1998, pp. 444-446
Citations number
25
Categorie Soggetti
Pharmacology & Pharmacy",Microbiology
Journal title
Antimicrobial agents and chemotherapy → ACNP
ISSN journal
00664804
Volume
42
Issue
2
Year of publication
1998
Pages
444 - 446
Database
ISI
SICI code
0066-4804(1998)42:2<444:TCCITU>2.0.ZU;2-3
Abstract
Two ganciclovir (GCV)-resistant human cytomegalovirus (HCMV) strains r ecovered from an AIDS patient (strain VR4990) and a heart transplant r ecipient (strain VR5474) showed a Cys607-->Tyr change in the UL97-enco ded phosphotransferase. No amino acid substitutions were observed in t he viral DNA polymerase. Marker transfer experiments showed marked red uction in GCV phosphorylation and drug susceptibility of the recombina nt HCMV strain VR4990rec2-1-1. These results further extend the region of the carboxy-terminal domain of the UL97 phosphotransferase involve d in GCV substrate recognition.