A. Vannerom et al., LUCIGENIN-ENHANCED AND LUMINOL-ENHANCED CHEMILUMINESCENCE IN TURKEY MONOCYTES, Journal of bioluminescence and chemiluminescence, 12(4), 1997, pp. 207-214
Monocytes from 10 week-old specific pathogen-free turkeys were isolate
d from peripheral blood by density centrifugation and assayed for thei
r oxidative activity by means of a luminometer. Chemiluminescence (CL)
properties after stimulation with different soluble and particulate s
timuli were compared in lucigenin-and luminol-enhanced assays. A disti
nct response could be measured with 12-phorbol 13-myristate acetate (P
MA) and Zymosan A, but only a weak signal was obtained with calcium io
nophore A23187. No oxidative activity could be induced with N-formyl-m
ethionyl-phenylalanine. Peak maxima for both lucigenin-and luminol-enh
anced CL were ranked: PMA > Zymosan A > calcium ionophore. The velocit
y of the lucigenin- and luminol-enhanced responses induced by calcium
ionophore were of similar magnitude, but the lucigenin-enhanced respon
ses of Zymosan A and PMA-stimulated monocytes were respectively about
5 and 10 times higher than those obtained in luminol-enhanced assays.
No peroxidase activity could be detected in the purified turkey monocy
tes. As luminol-enhanced CL primarily results from the peroxidase acti
vity, this lack of myeloperoxidase may explain the observed lower resp
onses to the different stimuli, in the presence of a luminol. In contr
ast, lucigenin-enhanced CL is not related to peroxidase activity, but
is a selective probe of oxidase activity. Irrespective of the myeloper
oxidase deficiency, different soluble and particulate stimuli induced
a significant and reproducible CL response in turkey monocytes, in the
presence of both chemiluminigenic probes, lucigenin and luminol. The
possibility of measuring the phagocyte oxygenation activity of turkey
monocytes represents a useful tool for the study of monocyte mediated
host defence in the turkey. (C) 1997 John Wiley & Sons, Ltd.