IMMOBILIZATION OF HORSERADISH-PEROXIDASE WITH A REGENERATED SILK FIBROIN MEMBRANE AND ITS APPLICATION TO A TETRATHIAFULVALENE-MEDIATING H2O2 SENSOR

Horseradish peroxidase (HRP) was immobilized onto a membrane of the re generated silk fibroin (RSF) from waste silk, The structure of the ble nd membrane of RSF and HRP was characterized by the use of IR spectra. A second generation of H2O2 sensor on the basis of the immobilized HR P was fabricated, in which tetrathiafulvalene acts as mediating electr on transfer between the immobilized enzyme and a glassy carbon electro de. Dependencies of pH and temperature on the H2O2 biosensor were chec ked by utilizing cyclic voltammetry. The sensor exhibits high sensitiv ity, good reproducibility and storage stability. (C) 1998 Published by Elsevier Science S.A.