MCI-154, A CARDIAC CA2-ACTIVATED FORCE BY INORGANIC-PHOSPHATE AND ACIDIC PH IN SKINNED FIBER OF GUINEA-PIG HEART( SENSITIZER, REVERSES THE DEPRESSION IN MAXIMAL CA2+)
Y. Kitada, MCI-154, A CARDIAC CA2-ACTIVATED FORCE BY INORGANIC-PHOSPHATE AND ACIDIC PH IN SKINNED FIBER OF GUINEA-PIG HEART( SENSITIZER, REVERSES THE DEPRESSION IN MAXIMAL CA2+), Cardiovascular drugs and therapy, 11(5), 1997, pp. 611-618
Intracellular accumulation of inorganic phosphate (Pi) and intracellul
ar acidosis, which occur in ischemic and hypoxic hearts, reduce the fo
rce of contraction by decreasing the responsiveness of contractile sys
tem to Ca2+. In the present study we investigated the effects of MCI-1
54, a Ca2+ sensitizer that can enhance crossbridge interaction, on the
decline in maximal Ca2+-activated force by Pi or acidic pH in skinned
fiber bundles of guinea pig hearts. MCI-154 can concentration-depende
ntly reverse the depression in maximal Ca2+-activated force (pCa 4.4)
by 20 mM Pi, which was not recovered by a higher concentration of Ca2 ion (pCa 4.0). The effects of MCI-154 were observed even at a concent
ration (0.01 M) at which the drug has no effect on the pCa 4.4-induced
maximal force in the absence of 20 mM Pi when given alone. MCI-154 in
hibited the rightward shift of the pCa-tension relationships, with a m
arked decrease of maximal force produced by 20 mM Pi or acidic pH (dec
rease in pH from 7.0 to 6.6). MCI-154 also improved the decline in max
imal Ca2+-activated force by 20 mM Pi under acidic pH, but the acidosi
s did not further decrease the effect of 20 mM Pi. Milrinone, a cyclic
AMP-dependent phosphodiesterase inhibitor, and pimobendan, another Ca
2+ sensitizer, did not improve the Pi-induced contractile failure. The
se results indicate that the Ca2+ sensitizer MCI-154 could reverse the
contractile failure induced by Pi and/or acidic pH in a skinned fiber
preparation via modulation of the strong crossbridge reaction with my
osin. MCI-154 may be a promising agent for myocardial contractile fail
ure, in which Pi and H+ progressively increase.