ESTRADIOL IN-VITRO MODULATES NA-DEPENDENT CA2+ UPTAKE BY SYNAPTIC PLASMA-MEMBRANE VESICLES OF RAT-BRAIN REGIONS()

Membrane vesicles loaded with [Na+], prepared from synaptosomal plasma membranes (SPM) of whole brains (WE), hippocampi (Hip) and caudate nu clei (NC) of female rats, were used to study Na+-dependent Ca2+ transp ort across SPM vesicles under the influence of 17 beta-estradiol (E-2) in vitro. In concentrations near to physiologic, E-2 significantly in creased Ca-45(2+) uptake by SPM vesicles from all the brain tissues in vestigated. The maximum increase was observed for WE (21%) and Hip (33 %) at 10(-9) mol/l, and for NC (31%) at 5 x 10(-9) mol/l of E-2. These results (a) confirm our earlier finding that E, in vitro modulates Na +-dependent Ca2+ transport across synaptosomal membrane in rat brain r egions, and (b) suggest Na+/Ca2+ exchange as principal mechanism of th e E-2-stimulated Na+-dependent Ca2+ uptake by membrane vesicles. The i nvolvement of any ATPases as possible mediators is discussed. (C) 1997 , Editrice Kurtis.