Being located in remote areas, alpine lakes are good indicators of reg
ional and global pollution, and are particularly sensitive to atmosphe
ric depositions. When situated in areas where acidic rocks dominate, t
hey are sensitive to acidification. In the framework of an internation
al project partially funded by the EU, a two-year study on zooplankton
was carried out on two lakes, Lake Paione Inferiore (LPI) and Lake Pa
ione Superiore (LPS), selected because of their susceptibility to acid
ification. In particular, LPS is permanently acidified, with pH rangin
g between 5.3 and 6.2, and LPI is acidified during the ice melt, when
pH drops to 6.1 units. In addition, LPI is subjected to further anthro
pogenic interference, since fish (Onchorynchus mykiss) have been repea
tedly introduced into the lake during the last thirty years. Literatur
e information is available on the species composition and the seasonal
dynamics of the zooplankton communities of the two lakes before these
anthropogenic disturbances took place. Previously, the two lakes had
a similar species composition and abundant zooplankton. An analysis of
the present-day situation reveals significant changes compared to the
past. Both acidification and the introduction of fish are responsible
for the marked alterations observed. By reconstructing of the past cl
adocera assemblages and by analysing the literature on lakes with diff
erent impacts of the two factors (pH and fish), we can estimate the re
lative importance of the two different anthropogenic disturbances on s
pecies composition and abundance of the zooplankton communities of the
two lakes.