A NEW FLAVONOL GLYCOSIDE GALLATE ESTER FROM ACER OKAMOTOANUM AND ITS INHIBITORY ACTIVITY AGAINST HUMAN IMMUNODEFICIENCY VIRUS-1 (HIV-1) INTEGRASE

Bioassay-directed chromatographic fractionation of an ethyl acetate ex tract of the leaves of Acer okamotoanum using HIV-1 integrase afforded a new acylated flavonol glycoside, quercetin 3-0-(2 '',6 ''-0-digallo yl)-beta-D-galactopyra (1), together with six known flavonol glycoside s and three known phenolic compounds. The structure of the new compoun d was determined by spectroscopic methods. The most active compounds w ere quercetin 3-O-(2 ''-galloyl)-alpha-L-arabinopyranoside (6) and 1, which exhibited IC50 values of 18.1 +/- 1.3 and 24.2 +/- 6.6 mu g/mL, respectively, against HIV-1 integrase.