A STUDY OF THE METABOLIC-DEGRADATION OF AN INSECT JUVENILE-HORMONE ANALOG USING DIFFERENT RADIOLABELING

The fate of an insect juvenile hormone analog applied to the insect bo dy of the flesh fly (Sarcophaga bullata) or the tsetse fly (Glossina p alpalis), respectively, was studied using three different radiolabeled positions in the parent biologically active compound 1. Several metab olites were found and analyzed. A mechanism of degradation of the appl ied molecule I as designed using a combination of several radioanalyti cal methods. A unique monitoring of the applied compound and its metab olites was provided, based on the different radiolabeling of the struc ture (cf. 1a-1c), and established their fate in an insect body during a 10-day experiment. A C-14 and H-3 radiolabeling, respectively, was e mployed to synthesize three different radiolabeled forms 1a-1e derived from the parent non-labeled 1. A combination of three different ways of radiolabeling resulted in an advantage in tracing the metabolic pat hway of degradation of the employed compound 1 in its radiolabeled for ms 1a-1c.