Jm. Aramini et al., SOFT-PULSED AL-27 QUADRUPOLAR CENTRAL TRANSITION NMR-STUDIES OF OVOTRANSFERRIN, Journal of magnetic resonance [1997], 129(2), 1997, pp. 111-114
We have employed soft(Gaussian) pulses to examine Al-27 NMR signals ar
ising from Al3+ bound to ovotransferrin. In addition to enhancing the
general detectability of Al-27 signals from a practical standpoint, th
is technique makes it possible to invert the central component of the
protein-bound Al-27 signals, providing a route for, to our knowledge,
the first inversion-recovery measurements on such systems. The suitabi
lity of this approach for gaining insights into the nature of metal io
n binding sites in large metallo-proteins is critically assessed. (C)
1997 Academic Press.