DIFFERENTIATION-SPECIFIC NUCLEAR MATRIX PROTEINS CROSS-LINKED TO DNA BY CIS-DIAMMINE DICHLOROPLATINUM

DNA-protein cross-linkages were performed in intact undifferentiated a nd differentiated HL60 cells by the action of cis-diammine dichloropla tinum. Total nuclear matrix proteins and DNA cross-linked nuclear matr ix proteins were resolved by two dimensional gel electrophoresis. The comparison of the electrophoretic patterns allowed the identification of st set of differentiation-induced nuclear matrix proteins cross-lin ked to DNA. One of these proteins binds cloned histone SAR sequences. Our results outline an experimental strategy for isolating and charact erizing nuclear matrix components that may play a fundamental role in the overall control and coordination of gene expression during differe ntiation. (C) 1998 Academic Press.