Sf. Ohandley et al., ORF186 REPRESENTS A NEW MEMBER OF THE NUDIX HYDROLASES, ACTIVE ON ADENOSINE(5')TRIPHOSPHO(5')ADENOSINE, ADP-RIBOSE, AND NADH, The Journal of biological chemistry, 273(6), 1998, pp. 3192-3197
orf186, a new member of the Nudix hydrolase family of genes, has been
cloned and expressed, and the protein has been purified and identified
as an enzyme highly specific for compounds of ADP. Its three major su
bstrates are adenosine(5') triphospho(5') adenosine, ADP-ribose, and N
ADH, all implicated in a variety of cellular regulatory processes, sup
porting the notion that the function of the Nudix hydrolases is to mon
itor the concentrations of reactive nucleoside diphosphate derivatives
and to help modulate their accumulation during cellular metabolism.