EFFLUENT TREATMENT OF PULP AND PAPER, AND TEXTILE INDUSTRIES USING IMMOBILIZED HORSERADISH-PEROXIDASE

Horseradish peroxidase immobilisation on several supports and its util isation for decontamination of effluent from paper and textile industr ies was evaluated. Among the supports, Amberlite IRA-400 ion exchange resin showed the best results for enzyme immobilisation, efficiency of colour removal and degradation of phenolic species from Kraft E1 effl uent(first alkaline extraction), bleaching mixture effluent and black liquor. Effluent decolorisations over 50% were obtained after 4 h of e nzymatic treatment, without significant adsorption of coloured species by the support. Decolorisation in pre-irradiated Kraft E1 effluent wa s kinetically more efficient than ina non-irradiated one. For this rea son, the combined photo-enzymatic; decolorisation procedure appears to be an efficient decontamination method with great potential for indus trial effluent treatment.