APPLICATION OF THE MULTICOLOR FISH TO INTERPHASE NUCLEI AND METAPHASESPREADS FOR SIMULTANEOUS EXAMINATION OF MONOSOMY-7 AND TRISOMY-8 AND TRISOMY-11 IN ACUTE MYELOCYTIC-LEUKEMIA (AML)

Authors
ACAR H ACAR A
Citation
H. Acar et A. Acar, APPLICATION OF THE MULTICOLOR FISH TO INTERPHASE NUCLEI AND METAPHASESPREADS FOR SIMULTANEOUS EXAMINATION OF MONOSOMY-7 AND TRISOMY-8 AND TRISOMY-11 IN ACUTE MYELOCYTIC-LEUKEMIA (AML), Clinical and laboratory haematology, 19(1), 1997, pp. 33-38
Citations number
25
Categorie Soggetti
Hematology
Journal title
Clinical and laboratory haematology → ACNP
ISSN journal
01419854
Volume
19
Issue
1
Year of publication
1997
Pages
33 - 38
Database
ISI
SICI code
0141-9854(1997)19:1<33:AOTMFT>2.0.ZU;2-1
Abstract
We have used the multi-colour (three) fluorescence in situ hybridizati on (FISH) technique based on the ratio labelling for the detection of monosomy 7 and trisomies 8 and 11 in 13 cases of acute myeloid leukaem ia (AML). Two out of the 13 AML cases showed monosomy 7 and two out of the remaining cases exhibited trisomy 8 in interphase nuclei. Three o f these results were confirmed by metaphase-FISH study. Trisomy 11 was not found either by the interphase FISH study or in the metaphase FIS H study. These results demonstrate the potential power of multi-colour FISH using ratio labelling to produce more fluorescein colour in inte rphase nuclei for the detection of aneuploidies in leukaemia.