A. Moutabarrik et al., THE EFFECT OF ORGAN PRESERVATION SOLUTIONS ON KIDNEY TUBULAR AND ENDOTHELIAL-CELLS, Transplant international, 11(1), 1998, pp. 58-62
Organ preservation solutions have primarily been tested in whole organ
animal models. In the current study, we have examined the effect of c
ommonly used organ preservation solutions on both kidney tubular and e
ndothelial cells. Primary human endothelial and kidney tubular cells w
ere incubated at 4 degrees C in the following solutions: 0.9 % saline
(NS), EuroCollins (EC), University of Wisconsin (UW), or Hank's balanc
ed salts with 5 % polyethylene glycol(PEG). Cell viability was assesse
d by colorimetric measurement of mitochondrial reduction of 3 4,5-dime
thylthiazol-2-yl)-2-,5-diphenyltatrazolium bromide (MTT) to purple 1-(
4,5-dimethylthiazol-2-yl)-3,5-diphenylformazan. After hypothermic stor
age, cells were incubated at 37 degrees C in media with MTT, and the a
mount of reduced formazan present was quantified. Endothelial cells pr
eserved in PEG displayed the best viability (P < 0.05). UW provided be
tter cellular viability than EC or NS (P < 0.05). Control endothelial
cells preserved in culture media at 37 degrees C displayed the highest
absorbance values (P < 0.01). For kidney tubular cells, UW and PEG pr
ovided the best cellular protection (P < 0.05). Control kidney tubular
cells cultured in complete media at 37 degrees C displayed the highes
t absorbance values (P < 0.01). Although the model presented here was
not part of a truly morphological study, it may be more reliable for t
he rapid assessment of preservation-induced cell injury than models pr
esented in previous morphological studies and may help in the developm
ent of improved preservation techniques.