Although two kinetically distinct evoked GABA(A), responses (GABA,(A,f
ast),,,, and GABA,(A,slow),,,,) have been observed in CA1 pyramidal ne
urons, studies of spontaneous IPSCs (sIPSCs) in these neurons have rep
orted only a single population of events that resemble GABA,(A,fast),,
,, in their rise and decay kinetics. The absence of slow sIPSCs calls
into question the synaptic basis of GABA,(A,slow).,,,,. We present evi
dence here that both evoked responses are synaptic in origin, because
two classes of minimally evoked, spontaneous and miniature IPSCs exist
that correspond to GABA,(A,fast),,,, and GABA(A,slow).,,,,, Slow sIPS
Cs occur infrequently, suggesting that the cells underlying these even
ts have a low spontaneous firing rate, unlike the cells giving rise to
fast sIPSCs. Like evoked GABA,(A,fast),,,, and GABA,(A,slow),,,,, fas
t and slow sIPSCs are modulated differentially by furosemide, a subtyp
e-specific GABA(A), antagonist, Furosemide blocks fast IPSCs by acting
directly on the postsynaptic receptors, because it reduces the amplit
ude of both miniature IPSCs and the responses of excised patches to ap
plied GABA. Thus, in the hippocampus, parallel inhibitory circuits are
composed of separate populations of interneurons that contact anatomi
cally segregated and pharmacologically distinct postsynaptic receptors
.