R. Srinivas et T. Panda, LOCALIZATION OF CARBOXYMETHYL CELLULASE IN THE INTERGENERIC FUSANTS OF TRICHODERMA-REESEI QM-9414 AND SACCHAROMYCES-CEREVISIAE NCIM-3288, Bioprocess engineering, 18(1), 1998, pp. 71-73
In order to convert cellulosic material to ethanol by single step proc
ess a chemofusion method has been followed between protoplasts of Tric
hoderma reesei, QM 9414, and the spheroplasts of Saccharomyces cerevis
iae, NCIM 3288, in the author's laboratory. The fusion was a success a
nd it was observed that endoglucanase was the key enzyme in the succes
s of the fusion. In the present study, characterization of the fusants
based on the endoglucanase synthesis, its localization and the distri
bution in the cells are described and compared with that of Trichoderm
a reesei, QM 9414, (wild type).