LOCALIZATION OF CARBOXYMETHYL CELLULASE IN THE INTERGENERIC FUSANTS OF TRICHODERMA-REESEI QM-9414 AND SACCHAROMYCES-CEREVISIAE NCIM-3288

In order to convert cellulosic material to ethanol by single step proc ess a chemofusion method has been followed between protoplasts of Tric hoderma reesei, QM 9414, and the spheroplasts of Saccharomyces cerevis iae, NCIM 3288, in the author's laboratory. The fusion was a success a nd it was observed that endoglucanase was the key enzyme in the succes s of the fusion. In the present study, characterization of the fusants based on the endoglucanase synthesis, its localization and the distri bution in the cells are described and compared with that of Trichoderm a reesei, QM 9414, (wild type).