CHARACTERIZATION AND DETERMINATION OF HUMAN URINARY KERATAN SULFATE

Authors
TOYODA H DEMACHI Y KOMORIYA S FURUYA N TOIDA T IMANARI T
Citation
H. Toyoda et al., CHARACTERIZATION AND DETERMINATION OF HUMAN URINARY KERATAN SULFATE, Chemical and Pharmaceutical Bulletin, 46(1), 1998, pp. 97-101
Citations number
24
Categorie Soggetti
Chemistry Medicinal",Chemistry,"Pharmacology & Pharmacy
Journal title
Chemical and Pharmaceutical Bulletin → ACNP
ISSN journal
00092363
Volume
46
Issue
1
Year of publication
1998
Pages
97 - 101
Database
ISI
SICI code
0009-2363(1998)46:1<97:CADOHU>2.0.ZU;2-L
Abstract
Keratan sulfate was isolated from normal human urine and was character ized by sugar compositional analysis and H-1-NMR spectroscopy. It was found that KS from human urine is classified as skeletal type (KS-II t ype chain) with an O-glycosidic linkage between galactosamine and seri ne (or threonine). H-1-NMR studies revealed that urinary KS is not a p roteoglycan but a polysaccharide (molecular weight is about 5 kDa). Th e quantitation of human urinary KS by HPLC showed that urinary KS is e xcreted at constant levels (0.07 +/- 0.015 mu g/mg creatinine).