4-(7-DIETHYLAMINOCOUMARIN-3-YL)BENZOYL CYANIDE (DACB-CN) - A HIGHLY SENSITIVE FLUORESCENT DERIVATIZATION REAGENT FOR ALCOHOLS IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
TAKECHI H GOTO Y MACHIDA M
Citation
H. Takechi et al., 4-(7-DIETHYLAMINOCOUMARIN-3-YL)BENZOYL CYANIDE (DACB-CN) - A HIGHLY SENSITIVE FLUORESCENT DERIVATIZATION REAGENT FOR ALCOHOLS IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Chemical and Pharmaceutical Bulletin, 46(1), 1998, pp. 159-162
Citations number
19
Categorie Soggetti
Chemistry Medicinal",Chemistry,"Pharmacology & Pharmacy
Journal title
Chemical and Pharmaceutical Bulletin → ACNP
ISSN journal
00092363
Volume
46
Issue
1
Year of publication
1998
Pages
159 - 162
Database
ISI
SICI code
0009-2363(1998)46:1<159:4C(-AH>2.0.ZU;2-S
Abstract
4-(7-Diethylaminocoumarin-3-yl)benzoyl cyanide (DACB-CN) was synthesiz ed as a new fluorescent derivatization reagent for alcohols for use in high-performance liquid chromatography. Saturated alcohols (C-8-C-22) were derivatized in good yields to give the corresponding fluorescent DACB-esters by treatment with DACB-CN. The DACB-esters of these alcoh ols were clearly separated on a reversed-phase HPLC column. The detect ion limit (S/N = 3) of cetyl alcohol, a test compound, was 1-2 fmol/l0 mu l.