PF1092A, PF1092B AND PF1092C, NEW NONSTEROIDAL PROGESTERONE-RECEPTOR LIGANDS PRODUCED BY PENICILLIUM-OBLATUM .1. TAXONOMY OF PRODUCING STRAIN, FERMENTATION, ISOLATION AND BIOLOGICAL-ACTIVITIES

Three new nonsteroidal progesterone receptor ligands, PF1092A, B and C , have been isolated from Penicillium oblatum. They were purified from the solid cultures of rice media using ethyl acetate extraction, sili ca gel and Sephadex LH-20 column chromatographies, and crystallization . All three ligands competitively inhibited [H-3]-progesterone binding to porcine uteri cytosol preparations with IC50 of 3.0 x 10 nM (PF109 2A), 2.2 x 10(2) nM (PF1092B) and 2.2 x 10(3) nM (PF1092C).