REGULATION OF GENES ASSOCIATED WITH ANGIOGENESIS, GROWTH, AND METASTASIS BY SPECIFIC P53 POINT MUTATIONS IN A MURINE MELANOMA CELL-LINE

K1735 murine melanoma cells transfected with p53 cDNAs bearing specifi c point mutations are metastatic in nude mice, whereas the parent and control-transfected cells are nonmetastatic. To determine whether p53 gene mutations regulate genes associated with angiogenesis, growth, an d metastasis, we examined expression of vascular endothelial growth fa ctor I, IGF-I receptor, epidermal growth factor insulin-like growth fa ctor I, IGF-I receptor, epidermal growth factor receptor, c-MET, and t hrombospondin 1 in K1735 cells transfected with one of four different mutant p53 cDNAs. Northern blot analysis demonstrated differential upr egulation of these genes in cells transfected with different mutant p5 3 cDNAs. Up-regulation of angiogenesis-, growth-, and metastasis-relat ed genes by mutant p53 may contribute to metastasis formation.