ENHANCEMENT OF SENSITIVITY TO CDDP IN HUMAN PHARYNGEAL CARCINOMA-CELLS BY H-7, A PROTEIN-KINASE INHIBITOR - POSSIBLE INVOLVEMENT OF CELLULAR GLUTATHIONE

Authors
NODA I SAITO H TANAKA N SUGIMOTO C FUJIEDA S
Citation
I. Noda et al., ENHANCEMENT OF SENSITIVITY TO CDDP IN HUMAN PHARYNGEAL CARCINOMA-CELLS BY H-7, A PROTEIN-KINASE INHIBITOR - POSSIBLE INVOLVEMENT OF CELLULAR GLUTATHIONE, Oncology Reports, 4(3), 1997, pp. 579-582
Citations number
25
Categorie Soggetti
Oncology
Journal title
Oncology Reports → ACNP
ISSN journal
1021335X
Volume
4
Issue
3
Year of publication
1997
Pages
579 - 582
Database
ISI
SICI code
1021-335X(1997)4:3<579:EOSTCI>2.0.ZU;2-U
Abstract
We investigated the synergetic effects of 1-(5-isoquinolinesulfonyl)-2 -methylpiperazine dihydrochloride (H-7) on cis-diamminedichloroplatinu m(II) (CDDP) cytotoxicity to KB cells, a human pharyngeal carcinoma ce ll line, and explored the possible mechanism whereby it exerted these effects. In vitro treatment with H-7 markedly decreased protein kinase C (PKC) activity in KB cells and significantly sensitized the cells t o CDDP. Treatment of KB cells with H-7 induced a dose-dependent deplet ion of intracellular glutathione (GSH) levels. These results suggest t hat the decreased GSH content induced by H-7 may be involved in sensit izing KB cells to CDDP.