Y. Sbihi et al., SPECIFIC RECOGNITION OF HYDATID CYST ANTIGENS BY SERUM IGG, IGE, AND IGA USING WESTERN-BLOT, Journal of clinical laboratory analysis, 11(3), 1997, pp. 154-157
Diagnosis of hydatid disease in humans relies on the detection of spec
ific antibodies against antigens of the metacestode from Echinococcus
granulosus, The specificity and sensitivity of current immunological t
echniques based on specific serum IgG rely on the way antigens are pur
ified. We used Western immunoblotting to detect specific IgG, IgE, and
IgA antibodies in serum from patients with hydatid disease using eith
er crude antigen preparations (total hydatid fluid), purified fraction
s enriched in Antigens 5 and B, and glycoproteins from hydatid fluid.
Depending on whether crude HF or purified antigen fractions were used,
IgG and IgE recognized specifically low-to-medium MW bands between 12
and 42 kDa. IgA recognized specifically 110 kDa band in crude hydatid
fluid and in the glycoprotein fraction of hydatid fluid, and a 42 kDa
band in ail antigen samples used. Besides the advantage of detecting
specific IgA in crude hydatid fluid, these results offer the possibili
ty of simplifying future immunological tests if specific secretory IgA
can be similarly detected. (C) 1997 Wiley-Liss, Inc.