IDENTIFICATION OF NEW MINISATELLITES LOCI IN ARABIDOPSIS-THALIANA

In order to characterize new GC-rich minisatellites present in the Ara bidopsis thaliana genome, a genomic library was screened at low string ency with a probe containing nine repealed-units of a minisatellite (C Ms1) previously identified, Both minisatellites and minisatellite-like elements were identified, The minisatellites, with a tandemly-repeate d structure, all contain the Arabidopsis thaliana-core sequence previo usly defined (Tourmente et al., 1994), Both minisatellite and minisate llite-like sequences occur in the Arabidopsis genome in low copy and a re weakly polymorphic between ecotypes. The genetic mapping of these m arkers has shown that they are dispersed on the genome, YACs clones of the CIC library carrying these minisatellites and minisatellite-like sequences were identified.