ACRIDINE-ORANGE LEUKOCYTE CYTOSPIN (AOLC) TEST AS AN IN-SITU METHOD FOR THE DIAGNOSIS OF CENTRAL VENOUS CATHETER (CVC)-RELATED SEPSIS IN ADULT RISK PATIENTS

Authors
VONBAUM H PHILIPPI P GEISS HK
Citation
H. Vonbaum et al., ACRIDINE-ORANGE LEUKOCYTE CYTOSPIN (AOLC) TEST AS AN IN-SITU METHOD FOR THE DIAGNOSIS OF CENTRAL VENOUS CATHETER (CVC)-RELATED SEPSIS IN ADULT RISK PATIENTS, Zentralblatt fur Bakteriologie, 287(1-2), 1998, pp. 117-123
Citations number
12
Categorie Soggetti
Microbiology
Journal title
Zentralblatt fur Bakteriologie → ACNP
ISSN journal
09348840
Volume
287
Issue
1-2
Year of publication
1998
Pages
117 - 123
Database
ISI
SICI code
0934-8840(1998)287:1-2<117:ALC(TA>2.0.ZU;2-#
Abstract
The AOLC method for the in-situ diagnosis of catheter-associated septi cemia was evaluated in adult intensive care patients. 55 blood samples and corresponding CVC tips were examined using the AOLC method, the s emiquantitative roll plate method and the broth immersion method. In 4 patients (7.3%), a CVC-related septicemia was diagnosed, 10 patients (18.2%) showed a colonisation of their central venous access. The AOLC method was positive in 50% of the cases with CVC-related septicemia a nd in 20% of the colonized patients. Thus, we do not recommend the AOL C test as a routine method for the diagnosis of suspected CVC-related infection but as a facultative additional diagnostic measure for certa in risk patients.