CONSTRUCTION OF RFLP-BASED MAPS OF FOXTAIL MILLET, SETARIA-ITALICA (L.) P. BEAUV

An RFLP-based map consisting of 160 loci was constructed in an interva rietal cross of foxtail millet [Setaria italica (L.) P. Beauv.], Longg u 25 x Pagoda Flower Green. The map comprises nine linkage groups, whi ch were aligned with the nine foxtail millet chromosomes using trisomi c lines, and spans 964 cM. The intraspecific map was compared to an in terspecific map, constructed in a S. italica x S. viridis cross. Both the order of the markers and the genetic distances between the loci we re highly conserved. Deviations from the expected 1:2:1 Mendelian segr egation ratios were observed in both the intra- and inter-specific pop ulations. The segregation data indicate that chromosome VIII in the Lo nggu 25 x Pagoda Flower Green cross carries a gene that strongly affec ts gamete fertility.