Sa. Burton et al., EVALUATION OF A GLUCOSE-OXIDASE PEROXIDASE METHOD FOR INDIRECT MEASUREMENT OF GLYCOGEN-CONTENT IN MARINE MUSSELS (MYTILUS-EDULIS), Journal of shellfish research, 16(2), 1997, pp. 435-439
A colorimetric method (glucose oxidase/peroxidase) for indirect measur
ement of glycogen concentrations in tissue homogenates of marine musse
ls (Mytilus edulis) was evaluated. This method uses a conversion of gl
ycogen to glucose by amyloglucosidase. Varying the buffer pH (4.5, 5.0
; 5.5) and the amyloglucosidase concentration (160, 80, 40, 20, 10, 5,
1, and 0.5 mg/mL) did not appreciably optimize glycogen concentration
. Coefficients of variation (n = 10) for mussel homopenates with mean
glycogen concentrations of 94 and 334 mg/dL had within-run values of 0
.75 and 0.96%, respectively. The between-run coefficients of variation
(n = 10) for the same homogenates were 2.10 and 1.10%, respectively.
When mean glycogen concentrations of thawed mussel homogenates were co
mpared with those of initial fresh homogenates, a significantly (p les
s than or equal to 0.05) lower glycogen concentration was seen in samp
les thawed after 1 day, but not in samples thawed after 1 h, 1 wk, or
1 me. Glycogen recovery percentages of 99.3, 99.0, and 95.6% were obta
ined with mixed solutions containing 103.8, 95.2, and 10.8 mg/dL glyco
gen, respectively. The lower limit of sensitivity for the procedure wa
s approximately 10 mg/dL. Because dilutions of a mussel homogenate wit
h a high glycogen concentration (413.1 mg/dL) gave observed results wi
thin 5% of expected results, the assay was considered to be linear to
at least 413.1 mg/dL. Glycogen concentrations based on analysis of wet
tissue and lyophilized samples from 20 mature mussels were compared,
resulting in a significant (p less than or equal to 0.05) correlation
coefficient of 0.52. An initial laboratory range (43-91 mg/g) for tiss
ue glycogen based on wet weights (3.9-12.4 g) was determined with 20 m
ature mussels during July from the Morell region, Prince Edward Island
, Canada. It was concluded that the colorimetric assay offered a relia
ble indication of tissue concentrations of glycogen in marine mussels
(M. edulis).