C-TERMINAL END OF V-SRC PROTEIN INTERACTS WITH PEPTIDE CODED BY GADD7ADAPT15-LIKE RNA IN 2-HYBRID SYSTEM/

The significant differences in the metastatic properties of hamster fi broblasts transformed by the Rous sarcoma virus (RSV) ere associated w ith mutations in the v-src carboxyterminal region, To identify the cap acity of this region for protein-protein interaction the two-hybrid sy stem was used. The cDNA clone (vseap1), producing the protein specific ally bound with the v-src C-terminal part in yeast cells in vivo and i n GST-fusion system in vitro was isolated, Vseap1 shared 68% of homolo gy with stressful agents induced RNA-gadd7/adapt15. Two vseap1 specifi c messenger RNAs were identified: 0.9-kbp RNA expressed in all transfo rmed cells and three times less in embryo fibroblasts; 3.1-kbp transcr ipt was deleted in the cells with suppressed v-src activity and H2O2 r esistance. (C) 1998 Federation of European Biochemical Societies.