PUTATIVE PROTEIN SERINE THREONINE KINASE GENES ARE LOCATED IN SEVERALPOSITIONS ON THE CHROMOSOME OF STREPTOMYCES-COELICOLOR A3(2)/

Seven DNA fragments containing putative protein serine/threonine kinas e genes were cloned from Streptomyces coelicolor A3(2) by hybridizatio n with a 165-bp amplified polymerase chain reaction product of pkaB co ntaining kinase subdomains VI, VII and VIII. Among them, the nucleotid e sequences of three fragments containing subdomains VI, VII and VIII were identical, while those of one fragment were identical with that o f afsK, another protein serine/threonine kinase gene. However, these k inase-like genes with very similar nucleotide sequences were distribut ed on the chromosome of S. coelicolor A3(2). (C) 1998 Federation of Eu ropean Microbiological Societies. Published by Elsevier Science B.V.