PROTECTIVE ROLES OF 2 ALUMINUM (AL)-INDUCED GENES, HSP150 AND SED1 OFSACCHAROMYCES-CEREVISIAE, IN AL AND OXIDATIVE STRESSES

We isolated two yeast cDNA clones whose transcripts are induced by alu minum (Al) metal stress. Partial nucleotide sequencing showed that one is the HSP150 gene encoding a secreted heat shock protein, and the ot her corresponds to the SED1 gene encoding a putative membrane protein. To clarify the biological functions of these genes, we analyzed the s ensitivity of gene-disrupted mutants to Al stress and to oxidative str esses. The Al tests indicated that the HSP150 protein served a basal p rotective role in Al stress, but SED1 did nor, both of the genes had p rotective roles for oxidative stresses. The results for the HSP150 gen e suggest that there is an overlap between Al ion stress, oxidative st ress and heat shock stress in yeast. (C) 1998 Published by Elsevier Sc ience B.V. All rights reserved.