IN-VITRO SUSCEPTIBILITY TESTING OF CANDID A SPECIES AGAINST FLUCONAZOLE USING THE MICRODILUTION TEST WITH ALAMAR-BLUE

The investigation of susceptibility of Candida species to fluconazole was performed in microdilution to a supplemented HR-medium. The suffic ient reproducibility of the test was verified using special control is olates and isolates of patients. The excellent applicability of the me thod in routine diagnostics was evaluated by in vitro testing of susce ptibility of 279 Candida isolates from patients being colonised or suf fering from endomycoses. The Candida species showed different suceptib ility against fluconazole: 96% of the C. albicans isolates were sensit ive, 55% of the C. glabrata isolates had a reduced sensitivity, and 26 % were resistant against fluconazole (MIC > 25 mu g/ml). C. krusei iso lates were highly resistant (9 of 11 strains).