ANTISERUM AGAINST NEUROPEPTIDE FF AUGMENTS VASOPRESSIN RELEASE IN CONSCIOUS RATS

We previously reported that centrally administered neuropeptide FF (NP FF) inhibited arginine vasopressin (AVP) release, In this study, immun oneutralization of central NPFF was performed to evaluate the role of endogenous NPFF in the regulation of AVP release. Intracerebroventricu lar (ICV) injection of antiserum against NPFF (Anti-NPFF) significantl y augmented the plasma AVP increase induced by hyperosmolality [intrap eritoneal injection of hypertonic. saline (600 mOsm/kg, 2% BW)] at 60 min after ICV injection compared with normal rabbit serum CNRS) (NRS: 4.20 +/- 0.30 pg/ml, Anti-NPFF: 5.83 +/- 0.46 pg/ml, p < 0.01), Anti-N PFF did not cause significant change in plasma osmolality, plasma volu me or arterial blood pressure, This evidence indicates that endogenous NPFF might be physiologically involved in osmoregulation of the plasm a AVP level through its inhibitory action. (C) 1998 Elsevier Science I nc.